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ToolboxLab TestingLive Watch (Targeted eRNA Assay)
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Lab Testing

Live Watch (Targeted eRNA Assay)

One species, one question: is it alive and active here now?

$1,750

One target species per kit. Not sure? Pick the invasive already found in nearby or connected lakes, or ask the Boathouse.

The Boathouse confirms every target against the lab's validated assays before your kit ships.

Name one target species above to add this assay to your cart.

Overview

A DNA detection tells you a species' genetic material was in the water. It does not tell you why. A zebra mussel signal at a busy boat launch could mean an established colony — or a contaminated boat that visited last week. Those two situations call for very different responses, and Live Watch is built to tell them apart.

Live Watch looks for the RNA of one named species, using a targeted assay. Because RNA begins degrading within minutes to hours of leaving a living cell, a positive result is strong evidence the organism is alive and active where you sampled, not just that its DNA passed through.

It also means eRNA cannot be collected the way eDNA is. Valid eRNA sampling requires high-volume pumped filtration to concentrate enough viable transcript signal, and immediate on-site chemical preservation to lock the snapshot before it breaks down. That is what the Osprey platform, supplied by our genomics partner, is built to do.

Use it to confirm, not to screen: after a Species Watch or Lake Census detection, to check whether a treated or removed population is still active, or where a response decision depends on knowing the organism is alive. Sold in sets of three sampling locations — typically the detection site and two nearby.

One of four genetic tests. “Census” reads the whole community; “Watch” looks for one named species. Lake Census and Species Watch read DNA; the two “Live” tests read RNA, which only living, active organisms produce.

  • Lake Census — DNA, broad: what lives here?
  • Species Watch — DNA, one species: is it here yet?
  • Live Census — RNA, broad: what is alive and active right now?
  • Live Watch — RNA, one species: is it alive and active here now?

Most lakes should start broad and narrow down: census first, then watch, then confirm. Which genetic test should your lake start with?

Note on methodology: eRNA analysis is not an EPA-approved method. It is a research-grade ecological tool, and results are not suitable for regulatory or compliance use.

What's detected & how

1 target · Targeted species eRNA assay

  • One named species (RNA), selected at checkout
    What's reported:
    Active RNA detected / Not detected, per location

You get

  • Active RNA detected / not detected for your target species at each of three locations, in Flora & Fauna
  • Signed laboratory report in Documents & Reports

How it's produced

Test type
Targeted species eRNA assay
Sample
Three sampling locations; high-volume pumped filtration with immediate on-site preservation (Osprey platform)
Analyzed by
Genomics partner laboratory
Method basis
Peer-reviewed molecular method; not EPA-approved
Turnaround
15–20 business days from lab receipt
Product category
  • Physical ProductShips to you
  • ConsumableLab testing
  • Availability

    Kit ships in 1–2 business days

  • Shipping

    Flat-rate to your front door. Shipping details

  • SKU

    E-RNA-002

Example result

Example result

Not detected at 3 of 3 locations

Not proof of absence.

No active RNA recovered on that day. Not proof of absence

Turnaround

Results are available in 15 - 20 business days of the lab receiving your samples.

What problem does this solve for our lake?

  • A DNA detection of an invasive species raises a question it can't answer: is there a living population, or did a contaminated boat or drifting water carry the signal in? The two call for very different responses, and getting it wrong is costly either way — an unnecessary alarm, or a missed infestation. Live Watch tests one species for signs of living, active presence, so your response rests on evidence rather than assumption.

What's included?

  • The Osprey platform ships to your site as two coordinated cases:
    • Osprey Control Case — stays on site for the duration of your program. Pump system, battery and charger, control electronics, tubing, sample mounts, flow meter, pre-filters, and labeled instructions.
    • Osprey Sample Case — travels between your site and the lab. Sample cartridges, shipping mounts, and pre-measured preservation buffer.
    • Return postage for the Sample Case
    • Three (3) sampling locations — the minimum order
    • Laboratory analysis by targeted eRNA assay for one target species, named at order and confirmed against the lab's validated assays, with full chain of custody
    • A lab report per location stating whether the target species' RNA was detected
    • Results loaded into your lake's account
    • Field workflow: cases arrive, run the pump per the provided guidelines, roughly 15 minutes of sampling per run, set the genetic signal with the buffer, ship the Sample Case back. One-time test, return both cases; ongoing program, keep the Control Case and cycle only the Sample Case.
    • Note on methodology: eRNA analysis does not run an EPA-approved method. It is a research-grade ecological tool, and results are not suitable for regulatory or compliance use.

What's not included

  • Sample collection. You run the platform; we supply everything needed to do it correctly.
  • Standard grab or syringe-filter collection. It will not concentrate enough viable transcript signal for a valid eRNA result.
  • Any species other than the one named at order. For the whole active community, order Live Census.
  • Species without a validated eRNA assay. The Boathouse will confirm your target before you order.
  • Single samples. Three locations is the minimum order.
  • Abundance or population estimates
  • Proof of absence. A non-detection means active RNA was not detected in this sample, at this place and time.
  • Regulatory or compliance determinations, including official invasive-species reporting
  • Treatment, remediation, or applicator services

Requirements

  • Immediate on-site preservation. RNA begins degrading within minutes to hours, so the buffering step happens at the water, not back at the house.
  • High-volume pumped filtration per the provided guidelines — roughly 15 minutes per sampling run
  • The Sample Case shipped back the same day it is filled
  • Somewhere secure and dry to keep the Control Case if you are running a multi-visit program
  • Sampling timed to the question. eRNA is a snapshot of a moment, so the moment has to be the one you care about.
  • A Plus, Lake Admin, or PRO subscription to have results loaded into your Lake Pulse account
  • A target species confirmed with the Boathouse against the lab's validated eRNA assays before you order
  • Questions about site selection, timing, or which genetic test fits your lake? Contact the Lake Pulse Boathouse before you order.
What you get back

Results

  • A clear result for your target species at each of your three locations: active RNA detected or not detected
  • Living-presence evidence to set against an earlier DNA detection — alive and active, or a signal that passed through
  • A dated entry in your lake's invasive-species record
  • Interpreted results are found in Flora & Fauna in the Lake Pulse Portal
  • The signed lab report is filed in Documents & Reports
  • Results are found in your lake's portal, accessible only to Plus, Lake Admin, and PRO subscription tiers

Pro tips

  • This is a confirmation test. Order it when a DNA result raises a question that only "is it alive?" can answer.
  • Sample the detection site and two sites nearby. That tells you both whether the organism is active and whether it is localized.
  • Sample soon after the DNA detection, while the question is live. Waiting a season changes what you are measuring.
  • Use it after a response. Four weeks after hand-pulling, a barrier, or a treatment, Live Watch shows whether the population is still active.
  • Keep the Control Case on site for a follow-up program. Repeat visits only cycle the Sample Case.
  • Report confirmed detections to your state agency, and call the Boathouse before announcing anything.
Results sync to the Portal for Plus, Lake Admin, and PRO subscribers

Results from this product land in your lake's shared record in the Portal — next to everything else your lake has ever measured, instead of in an inbox.

Compare subscriptions

About these results

Every result shows the date and time each sample was collected. eDNA and eRNA detect genetic material, not organisms directly. DNA can persist after an organism has left or died, and can arrive from upstream or on boats, birds, or bait. Read counts reflect relative genetic signal, not the number, size, or biomass of organisms. Species names depend on reference-library coverage; some detections resolve only to genus or family. A detection is evidence to follow up, not confirmation that a population is established, and a non-detection does not prove absence. These are not EPA-approved methods and are not a regulatory determination. Results can help you make decisions about safe swimming and water use, but no result is definitive for every place and time. It is always good practice to check posted signs and your state or local health authorities.

RNA indicates recent biological activity. A not-detected result means no active RNA was recovered at that location on that day.

Questions on this?

Our team can help you choose the right solution for your lake, explain how results sync to your Portal, or point you to the right subscription.

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